VPS37B Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-06284
![Western Blot: VPS37B Antibody [NBP3-06284]](https://resources.rndsystems.com/images/products/VPS37B-Antibody-Western-Blot-NBP3-06284-img0001.jpg "Western Blot: VPS37B Antibody [NBP3-06284]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human VPS37B fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for VPS37B Antibody - BSA Free
Western Blot: VPS37B Antibody [NBP3-06284]
Western Blot: VPS37B Antibody [NBP3-06284] - Anti-VPS37B rabbit polyclonal antibody at 1:500 dilutionLane A: RT4 Whole Cell LysateLane B: U251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.)![Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284]](https://resources.rndsystems.com/images/products/VPS37B-Antibody-Immunohistochemistry-Paraffin-NBP3-06284-img0004.jpg "Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284]")
Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284]
Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284] - Immunochemical staining of human VPS37B in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.![Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284]](https://resources.rndsystems.com/images/products/VPS37B-Antibody-Immunohistochemistry-Paraffin-NBP3-06284-img0003.jpg "Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284]")
Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284]
Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284] - Immunochemical staining of human VPS37B in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.![Immunoprecipitation: VPS37B Antibody [NBP3-06284]](https://resources.rndsystems.com/images/products/VPS37B-Antibody-Immunoprecipitation-NBP3-06284-img0002.jpg "Immunoprecipitation: VPS37B Antibody [NBP3-06284]")
Immunoprecipitation: VPS37B Antibody [NBP3-06284]
Immunoprecipitation: VPS37B Antibody [NBP3-06284] - VPS37B was immunoprecipitated using:Lane A:0.5 mg U251 MG Whole Cell Lysate4 uL anti-VPS37B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-VPS37B rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :35 kDaApplications for VPS37B Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VPS37B
Alternate Names
ESCRT-I complex subunit VPS37B, FLJ12750, hVps37B, vacuolar protein sorting 37 homolog B (S. cerevisiae), vacuolar protein sorting 37B, vacuolar protein sorting 37B (yeast), vacuolar protein sorting-associated protein 37B
Gene Symbol
VPS37B
Additional VPS37B Products
Product Documents for VPS37B Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VPS37B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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