VPS45 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-98931

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

A synthetic peptide from rat VPS45 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous with the corresponding sequence in mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS45 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - Rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG at a concentration of 10 ug/ml.
Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody - Azide and BSA Free [NB100-98931]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Applications for VPS45 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Frozen

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Sterilized water

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VPS45

FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.; SUBUNIT: Interacts with STX6 and ZFYVE20. SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network. TISSUE SPECIFICITY: Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain.

Alternate Names

H1, H1VPS45, hlVps45, h-vps45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45A (yeast), vacuolar protein sorting-associated protein 45, VPS45A, VPS45AVPS54A, VPS45B, VSP45, VSP45A

Entrez Gene IDs

22365 (Mouse); 64516 (Rat)

Gene Symbol

VPS45

UniProt

Additional VPS45 Products

Product Documents for VPS45 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for VPS45 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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