![Western Blot: VPS50 Antibody [NBP1-78217]](https://resources.rndsystems.com/images/products/VPS50-Antibody-Western-Blot-NBP1-78217-img0002.jpg "Western Blot: VPS50 Antibody [NBP1-78217]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 914 and 964 of human Coiled-Coil Domain Containing 132 using the numbering given in entry NP_060137.2 (GeneID 55610).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VPS50 Antibody
Western Blot: VPS50 Antibody [NBP1-78217]
Western Blot: VPS50 Antibody [NBP1-78217] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-CCDC132 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC132 was also immunoprecipitated by rabbit anti-CCDC132 antibody NBP1-78216, which recognizes an upstream epitopeApplications for VPS50 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 mcg/mg
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: VPS50
Alternate Names
CCDC132, coiled-coil domain containing 132, coiled-coil domain-containing protein 132, EARP/GARPII Complex Subunit VPS50, KIAA1861, Syndetin, VPS50, EARP/GARPII Complex Subunit, VPS54L
Gene Symbol
VPS50
UniProt
Additional VPS50 Products
Product Documents for VPS50 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VPS50 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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