WRN Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20914

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WRN Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human WRN. The exact sequence is proprietary.

Localization

Nucleus; nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WRN Antibody - BSA Free

Western Blot: WRN Antibody [NBP2-20914]

Western Blot: WRN Antibody [NBP2-20914]

Western Blot: WRN Antibody [NBP2-20914] - Sample (30 ug of whole cell lysate) A: A431 B: Molt-4 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: WRN Antibody [NBP2-20914]

Immunocytochemistry/ Immunofluorescence: WRN Antibody [NBP2-20914]

Immunocytochemistry/Immunofluorescence: WRN Antibody [NBP2-20914] - Analysis of paraformaldehyde-fixed HeLa, antibody at 1:100.

Applications for WRN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Western Blot

1:500 - 1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-20914 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 40% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WRN

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq]

Long Name

Werner syndrome ATP-dependent helicase

Alternate Names

EC 3.1.-.-, Exonuclease WRN, RecQ protein-like 2, RecQ3, RECQL2

Gene Symbol

WRN

UniProt

Q14191 (Human)

Additional WRN Products

Product Documents for WRN Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WRN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for WRN Antibody - BSA Free (1)

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  • WRN Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 07/25/2019
    First column : siRNA CTRL 2,3,4 colums : siRNA WRN for 2 different cell lines
    40ug protein 1/1000 dilution Ab incubation 4°C ON
    WRN Antibody - BSA Free NBP2-20914

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