Xanthine Oxidase Activity Assay Kit (Fluorometric)

Novus Biologicals | Catalog # NBP3-24547

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and animal tissue

Sensitivity

0.01 U/L

Assay Range

0.01 - 1.2 U/L
View all Xanthine Oxidase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Fluorometric)

Species

Non-species specific

Description

Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm)
Assay time: 25 min

Precision

Intra-Assay Precision (Precision within an assay) %CV 4.1

Inter-Assay Precision (Precision between assays) %CV 9

Scientific Data Images for Xanthine Oxidase Activity Assay Kit (Fluorometric)

Xanthine Oxidase Activity Assay Kit (Fluorometric)

Xanthine Oxidase Activity Assay Kit (Fluorometric) [NBP3-24547] -

Xanthine Oxidase Activity Assay Kit (Fluorometric) [NBP3-24547] - Standard Reference Curve
Xanthine Oxidase Activity Assay Kit (Fluorometric)

Xanthine Oxidase Activity Assay Kit (Fluorometric) [NBP3-24547] -

Xanthine Oxidase Activity Assay Kit (Fluorometric) [NBP3-24547] - Assay Example

Kit Contents for Xanthine Oxidase Activity Assay Kit (Fluorometric)

  • Black Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Probe Solution
  • Reagent 3: Enzyme Reagent
  • Reagent 4: Substrate
  • Reagent 5: 2 mmol/L H2O2 Standard Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Xanthine Oxidase

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. (provided by RefSeq)

Long Name

Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase

Alternate Names

EC 1.17.3.2, XD, XDH, XDHA, XOR)

Gene Symbol

XDH

Additional Xanthine Oxidase Products

Product Documents for Xanthine Oxidase Activity Assay Kit (Fluorometric)

Certificate of Analysis

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Product Specific Notices for Xanthine Oxidase Activity Assay Kit (Fluorometric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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