Recombinant Human XLF His Protein

Name: Recombinant Human XLF His Protein
Brand: Novus Biologicals
SKU: NBP2-23291
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-23291

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all XLF Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-224 of Human XLF

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEELEQG LLMQPWAWLQ LAENSLLAKV FITKQGYALL VSDLQQVWHE QVDTSVVSQR AKELNKRLTA PPAAFLCHLD NLLRPLLKDA AHPSEATFSC DCVADALILR VRSELSGLPF YWNFHCMLAS PSLVSQHLIR PLMGMSLALQ CQVRELATLL HMKDLEIQDY QESGATLIRD RLKTEPFEEN SFLEQFMIEK LPEACSIGDG KPFVMNLQDL YMAVTTQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

27.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human XLF His Protein

SDS-PAGE: Recombinant Human XLF His Protein [NBP2-23291]

SDS-Page: Recombinant Human XLF Protein [NBP2-23291]

Formulation, Preparation, and Storage

NBP2-23291

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XLF

Non-homologous end-joining factor 1, also known as NHEJ1, belongs to the XLF family. NHEJ1 was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Patients with NHEJ1 mutations also have immunodeficiency due to a defect in V(D)J recombination, which utilizes NHEJ to promote immune system diversity. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the ligation step of NHEJ. Recombinant human NHEJ1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Cernunnos, non-homologous end-joining factor 1, nonhomologous end-joining factor 1, Protein cernunnos, XLFFLJ12610, XRCC4-like factor

Gene Symbol

NHEJ1

Additional XLF Products

Product Documents for Recombinant Human XLF His Protein

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Product Specific Notices for Recombinant Human XLF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human XLF His Protein

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