Recombinant Human YARS His Protein

Novus Biologicals | Catalog # NBP1-72303

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all YARS Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-528 of Human YARS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

61.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human YARS His Protein

SDS-PAGE: Recombinant Human YARS His Protein [NBP1-72303]

SDS-PAGE: Recombinant Human YARS His Protein [NBP1-72303]

SDS-Page: Recombinant Human YARS Protein [NBP1-72303] - 15% SDS-PAGE (3ug)
SDS-PAGE: Recombinant Human YARS His Protein [NBP1-72303]

SDS-PAGE: Recombinant Human YARS His Protein [NBP1-72303]

SDS-Page: Recombinant Human YARS Protein [NBP1-72303] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-72303
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YARS

Tyrosyl-tRNA synthetase, also known as YARS, belongs to the class I tRNA synthetase family. YARS is essential enzyme that catalyze the aminoacylation of tRNATrp with tryptophan, an essential function of the cell's protein synthesis machinery. Expression of YARS is highly stimulated in human cells by the addition of IFN-gamma. Recombinant human YARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CMTDIC, EC 6.1.1, EC 6.1.1.1, Tyrosyl--tRNA ligase, tyrosyl-tRNA synthetase, tyrosyl-tRNA synthetase, cytoplasmic, TYRRS, YRStyrRS, YTStyrosine tRNA ligase 1, cytoplasmic

Gene Symbol

YARS1

Additional YARS Products

Product Documents for Recombinant Human YARS His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human YARS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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