Z-FR-AMC Fluorogenic Peptide Substrate

Substrate for Papain
Catalog # Availability Size / Price Qty
ES009
Product Details
Citations (2)
FAQs
Reviews (1)

Z-FR-AMC Fluorogenic Peptide Substrate Summary

Activity
Hydrolysis of Arg-AMC amide bond releases 7-amino-4-methyl coumarin (AMC), a highly fluorescent group. It is an excellent substrate for papain, a cysteine protease often used in characterization of cysteine protease inhibitors.
Source

Z-Phe-Arg-AMC. Z: N-carbobenzyloxy; 7-Amino-4-methylcoumarin.
Predicted Molecular Mass
612.6 Da

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES009

Formulation Supplied as a stock solution in dimethyl sulfoxide (DMSO) with a concentration of 100 mg/mL by mass or 157.4 mM with consideration of the peptide content.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: No Stability Data Storage is set to N/A for ProdCode
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Citations for Z-FR-AMC Fluorogenic Peptide Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: N/A
    Sample Types: Recombinant Protein
  2. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005;444(1):7-14.
    Species: N/A
    Sample Types: Recombinant Protein

FAQs

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Reviews for Z-FR-AMC Fluorogenic Peptide Substrate

Average Rating: 5 (Based on 1 Review)

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Z-FR-AMC Fluorogenic Peptide Substrate
By Anonymous on 10/26/2020
Application: Enzymatic activity in vitro
Reason for Rating: Protease activity assay for cst6 protein. The curve is excellent.