Z-FR-AMC Fluorogenic Peptide Substrate

Substrate for Papain
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R&D Systems Fluorogenic Peptide Substrates
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Product Details
Citations (5)
Reviews (1)

Z-FR-AMC Fluorogenic Peptide Substrate Summary

Product Specifications

Hydrolysis of Arg-AMC amide bond releases 7-amino-4-methyl coumarin (AMC), a highly fluorescent group. It is an excellent substrate for papain, a cysteine protease often used in characterization of cysteine protease inhibitors.

Z-Phe-Arg-AMC. Z: N-carbobenzyloxy; 7-Amino-4-methylcoumarin.
Predicted Molecular Mass
612.6 Da

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Citations for Z-FR-AMC Fluorogenic Peptide Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Systematic Identification of Genomic Markers for Guiding Iron Oxide Nanoparticles in Cervical Cancer Based on Translational Bioinformatics
    Authors: H Zhou, J Tian, H Sun, J Fu, N Lin, D Yuan, L Zhou, M Xia, L Sun
    International Journal of Nanomedicine, 2022-06-29;17(0):2823-2841.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation
    Authors: E Mittler, T Alkutkar, RK Jangra, K Chandran
    MBio, 2021-01-12;12(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  3. The abnormal accumulation of heparan sulfate in patients with mucopolysaccharidosis prevents the elastolytic activity of cathepsin V
    Authors: T Chazeirat, S Denamur, KK Bojarski, PM Andrault, D Sizaret, F Zhang, A Saidi, M Tardieu, RJ Linhardt, F Labarthe, D Brömme, SA Samsonov, G Lalmanach, F Lecaille
    Carbohydrate polymers, 2020-10-20;253(0):117261.
    Species: N/A
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  4. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
  5. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005-10-21;444(1):7-14.


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Reviews for Z-FR-AMC Fluorogenic Peptide Substrate

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Z-FR-AMC Fluorogenic Peptide Substrate
By Anonymous on 10/26/2020
Application: Enzymatic activity in vitro
Reason for Rating: Protease activity assay for cst6 protein. The curve is excellent.