ZIC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86833

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZIC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGINPFADGMGAFKLNPSSHELASAGQTAFTSQAPGYAAAAALGHHHHPGHVGSYSSAAFNSTRDFLFRNRGFGDAAAAASAQHSLFAASAGGFGGPHGHTDAAGHLLFPGLHEQA

Marker

Ectoderm Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ZIC1 Antibody - BSA Free (NBP1-86833) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-ZIC1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ZIC1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody [NBP1-86833]

Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody [NBP1-86833]

Immunocytochemistry/Immunofluorescence: ZIC1 Antibody [NBP1-86833] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.
Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833]

Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human lymph node shows no positivity in germinal center cells as expected.

Applications for ZIC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZIC1

The Zic-1 gene, which encodes a zinc finger protein, is expressed in the developing or matured central nervous system in a highly restricted manner. The Zic gene is expressed in granule cells that make synaptic contact with Purkinje cells. Clearly Zic-1 is a gene critical to cerebellar pattern formation. The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm. Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family. By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation. The Zic-1 gene has been mapped to chromosome 9 in mouse. The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis. The temporal profile of mRNA expression differs for each of the Zic gene products. The Drosophila odd-paired gene is highly homologous to the Zic gene family.

Long Name

Zic Family Member 1

Alternate Names

ZNF201

Entrez Gene IDs

7545 (Human)

Gene Symbol

ZIC1

UniProt

Q15915

Additional ZIC1 Products

Product Documents for ZIC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZIC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ZIC1 Antibody - BSA Free

Customer Reviews for ZIC1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ZIC1 Antibody - BSA Free and earn rewards!

Have you used ZIC1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies