Zymosan, TLR2 Ligand

Novus Biologicals | Catalog # NBP2-26233

Novus Biologicals
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Key Product Details

Species

Human

Applications

Functional Assay, Inhibition Activity

Product Summary for Zymosan, TLR2 Ligand

Zymosan is a yeast cell wall derivative that is composed of b-glucans, mannans, mannoproteins and chitin. Zymosan is a known activator of TLR2, of which inflammatory responses to this particle are synergistically generated by collaboration with Dectin-1 and result in NF-kB-induced inflammatory cytokine production and phagocytosis1. Zymosan is also known to directly bind TLR2. Product Validation The data shows that Zymosan activated the TLR2 cells but not the control cells.

Product Specifications

Applications

In vitro assay (reported in scientific literature (PMID 27351455))
In vivo assay (reported in scientific literature (PMID 25846753))
Ligand Activation (reported in scientific literature (PMID 25957979))
Functional (Inhibition) (reported in scientific literature (PMID 25846753))

Application Notes

This product is useful for Activation of Toll-like Receptor 2 (TLR2).Stimulation of TLR2 has been reported with 1-10 ug/ml. Note: TLR2 activation at this range of concentrations of Zymosan has also been observed in our product validation tests, please see the image.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25957979)

Scientific Data Images for Zymosan, TLR2 Ligand

Zymosan, TLR2 Ligand [NBP2-26233] - Ligand activity of Zymosan. The NF-kB/SEAPorter/HEK 293 and TLR2/NFkB/SEAPorter/ HEK 293 cell line cell lines were plated in a 96-well plate at 5 x 10^4 cells/well for 16 h. Cells were then stimulated with Zymosan for 24h. The cell culture supernatants were analyzed for SEAP activity using SEAPorter Assay Kit.

Formulation, Preparation, and Storage

Preparation Method

To prepare a 50mg/ml stock solution, mix 50mg Zymosan with 1ml ethanol in a sterile non-pyrogenic vial by vortexing. For further dilution, use sterilized endotoxin-free water or PBS. The stock solution is insoluble and remains opaque. Vortex before use.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Background: Zymosan, TLR2 Ligand

Alternate Names

Zymosan, TLR2 Ligand

Additional Zymosan, TLR2 Ligand Products

Product Documents for Zymosan, TLR2 Ligand

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Zymosan, TLR2 Ligand

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for Zymosan, TLR2 Ligand

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FAQs for Zymosan, TLR2 Ligand

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  • Q: What is the aproximate molecular weight of the zymosan TLR2 Ligand, NBP2-26233?

    A: Since Zymosan is a polymer and therefore can be variable (a range of molecular weights) based upon the number of subunits in the polymer. The base unit is about 15 kDa with 3 subunits A/B/C.

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