Markers and Methods to Verify Mesenchymal Stem Cell Identity, Potency, and Quality

Mesenchymal stem cells (MSCs) are multipotent cells that are functionally defined by their capacity to self-renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes. Translation of MSC-based therapies has been confounded by MSC population heterogeneity as well as non-standardized methods for their definition and characterization. This white paper begins by defining MSCs and discussing the evolution of its nomenclature. It then provides a detailed description of MSC markers and how they may vary by tissue source and species. Ultimately, the white paper discusses how to standardize MSC characterization by selecting markers for isolation, characterization, and validation.

Key Topics

• Defining Mesenchymal Stem Cells
• Established and New MSC Markers
• Methods for MSC Isolation
• MSC Marker Variation by Species
• Methods for MSC Identification and Characterization
• Media Definitions for MSC Expansion