Pluripotent Stem Cell Verification: 3 Ways to Utilize a Single Well of a 24-well Plate

ABSTRACT

Pluripotent stem cell verification is a crucial process both for newly established cell lines as well as during routine culture. Here, we describe three methods for easy stem cell verification using the small sample size of a 24-well plate:

1. Verification by Antibody-Based Array: Membrane-based multiplex antibody array analysis verifies the expression of 15 different pluripotent and differentiated cell markers from the lysate of a single well of a 24-well plate, in less time than it takes to perform a single Western blot.
2. Verification by Live Cell Imaging: GloLIVE™ live-cell imaging antibodies allow for quick verification of pluripotent marker expression in live cells within 30 minutes.
3. Verification by Tri-lineage Differentiation: Directed tri-lineage differentiation functionally verifies cell potency within 5 days.

Together these tools assess cell quality with ease and reliability, leading to consistency among experiments while saving time, money, and reagents.

BG01V human embryonic stem cells are licensed from ViaCyte, Inc.

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