Culture of Mouse Enteric Organoids using Cultrex Basement Membrane Extract
This protocol is intended as a guide only, for full experimental details please read the reference provided.
This protocol outlines a method for the culture of organoid progenitor cells from normal and healthy mouse gastric, small intestine, or colon tissues using Cultrex® Organoid Qualified Basement Membrane Extract as a scaffold. Some specific tips and considerations you can find in this protocol include:
- Considerations and tips for starting enteric organoid cultures from frozen stock
- How Cultrex Organoid Qualified BME performs as a scaffold to support enteric mouse organoid cultures
- Maintenance of mouse enteric organoid cultures, including tips for successful passaging
- Cryobanking and preservation of enteric organoids for analysis and future experiments
Related Resources
- View Optimizing Organoid Culture Conditions: The Importance of Growth Factor Bioactivity and Reagent Consistency Application Note
- View All Cultrex Products
- View All Resources and Reagents for Organoid Research
- Request a Sample of Cultrex™ BME Matrix
- Learn more about BME Matrices
- Organoid and 3-D Cell Culture Protocols
