Culture of Mouse Enteric Organoids using Cultrex Basement Membrane Extract

This protocol outlines a method for the culture of organoid progenitor cells from normal and healthy mouse gastric, small intestine, or colon tissues using Cultrex® Organoid Qualified Basement Membrane Extract as a scaffold. Some specific tips and considerations you can find in this protocol include:

  • Considerations and tips for starting enteric organoid cultures from frozen stock
  • How Cultrex Organoid Qualified BME performs as a scaffold to support enteric mouse organoid cultures
  • Maintenance of mouse enteric organoid cultures, including tips for successful passaging
  • Cryobanking and preservation of enteric organoids for analysis and future experiments

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