Optimizing Organoid Culture Conditions: The Importance of Growth Factor Bioactivity and Reagent Consistency

Organoids are emerging as powerful model systems for investigating human organ development, modeling diseases, screening potential drugs, and exploring personalized medicine. While many scientists are now interested in using organoid models for their research, identifying the ideal conditions for culturing organoids can be difficult. This is primarily due to complex, multi-component cultures, technically advanced protocols, and variabilities that may occur from one experiment to the next. As a result, researchers frequently spend a considerable amount of time and resources trying to optimize the conditions needed to support robust, reproducible organoid expansion.

Read this application note to learn the importance of growth factor bioactivity and lot-to-lot consistency for achieving optimal, reproducible organoid culture conditions and see why so many scientists choose R&D Systems proteins to help advance their organoid research.