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Observation: High background level
| Problem | Corrective Action |
|---|
| Insufficient washing | Wash per protocol being sure to remove all Wash Buffer from wells before addition of next component |
| Contamination with alkaline phosphatase | Keep work area clean and free of alkaline phosphatase |
Observation: Poor precision
| Problem | Corrective Action |
|---|
| Plate not washed before use | Wash per protocol |
| RNase contamination | Use RNase-free technique |
| Pipetting error | Use a new pipet tip for each pipetting step and use proper technique |
Observation: No signal for positive control
| Problem | Corrective Action |
|---|
| Component or step omitted | Read protocol thoroughly before repeating the assay |
| RNase contamination | Use RNase-free technique |
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