Coupling Rates for 10 Minute Kinase Reactions

The following table lists the coupling rates for 10 minute kinase reactions coupled with Recombinant Mouse CD39L2. The coupling rates are for 50 μL reactions carried out at room temperature in a buffer consisting of 25 mM HEPES, 10 mM CaCl₂, 10 mM MgCl₂, 150 mM NaCl, pH 7.0. The coupling rates were calculated using a specific rate constant of 97.78 nmol/min/μg/mM.

NOTE: An * represents the optimal range for coupling rates in order to achieve the maximum signal-to-noise ratio for the phosphatase-coupled kinase reaction.

For more detailed technical information, please also see: Wu, Z.L. (2011) PLoS ONE 6(8):e23172.

Coupling Rates for 10 Minute Kinase Reactions

ATP (μM)	CD39L2 (μg)
ATP (μM)	0.025 μg	0.050 μg	0.075 μg	0.100 μg	0.125 μg	0.150 μg	0.175 μg	0.200 μg
50 μM	0.183	0.323	0.431*	0.515*	0.581*	0.634	0.677	0.712
100 μM	0.174	0.309	0.415*	0.498*	0.564*	0.618	0.662	0.697
150 μM	0.168	0.299	0.403*	0.486*	0.552*	0.606	0.650	0.686
200 μM	0.163	0.291	0.394	0.475*	0.542*	0.596*	0.640	0.677
250 μM	0.159	0.285	0.386	0.467*	0.533*	0.587*	0.632	0.669
300 μM	0.155	0.279	0.379	0.460*	0.525*	0.580*	0.625	0.662
400 μM	0.149	0.269	0.367	0.447*	0.512*	0.567*	0.612	0.650
500 μM	0.144	0.262	0.357	0.436*	0.501*	0.556*	0.601	0.640
600 μM	0.140	0.255	0.349	0.427*	0.492*	0.546*	0.592*	0.631
700 μM	0.137	0.249	0.342	0.419*	0.483*	0.537*	0.583*	0.622
800 μM	0.133	0.244	0.335	0.411*	0.476*	0.530*	0.576*	0.615
900 μM	0.130	0.239	0.329	0.405*	0.468*	0.522*	0.568*	0.608
1000 μM	0.128	0.234	0.324	0.399	0.462*	0.516*	0.562*	0.601
1100 μM	0.125	0.230	0.319	0.393	0.456*	0.510*	0.556*	0.595*
1200 μM	0.123	0.227	0.314	0.388	0.450*	0.504*	0.550*	0.590*
1300 μM	0.121	0.223	0.310	0.383	0.445*	0.499*	0.544*	0.584*
1400 μM	0.119	0.220	0.305	0.378	0.440*	0.493*	0.539*	0.579*
1500 μM	0.118	0.217	0.302	0.374	0.436*	0.489*	0.535*	0.574*
1600 μM	0.116	0.214	0.298	0.370	0.431*	0.484*	0.530*	0.570*
1700 μM	0.114	0.212	0.295	0.366	0.427*	0.480*	0.525*	0.565*
1800 μM	0.113	0.209	0.291	0.362	0.423*	0.476*	0.521*	0.561*
1900 μM	0.111	0.207	0.288	0.358	0.419*	0.472*	0.517*	0.557*
2000 μM	0.110	0.204	0.285	0.355	0.415*	0.468*	0.513*	0.553*
2100 μM	0.109	0.202	0.282	0.352	0.412*	0.464*	0.510*	0.549*
2200 μM	0.108	0.200	0.280	0.349	0.408*	0.460*	0.506*	0.546*
2300 μM	0.106	0.198	0.277	0.346	0.405*	0.457*	0.502*	0.542*
2400 μM	0.105	0.196	0.275	0.343	0.402*	0.454*	0.499*	0.539*
2500 μM	0.104	0.194	0.272	0.340	0.399	0.451*	0.496*	0.536*
2600 μM	0.103	0.193	0.270	0.337	0.396	0.448*	0.493*	0.532*
2700 μM	0.102	0.191	0.268	0.335	0.393	0.445*	0.490*	0.529*
2800 μM	0.101	0.189	0.266	0.332	0.391	0.442*	0.487*	0.526*
2900 μM	0.100	0.188	0.264	0.330	0.388	0.439*	0.484*	0.523*
3000 μM	0.100	0.186	0.261	0.327	0.385	0.436*	0.481*	0.521*

Coupling Rates for 10 Minute Kinase Reactions

Coupling Rates for 10 Minute Kinase Reactions

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