An reaction mixture containing CD39L2 and the kinase is added to each well of a 96-well microplate.
A substrate mixture containing ATP and the acceptor substrate is then added to each well of the microplate to initiate the kinase reaction.
The Malachite Green Phosphate Detection Reagents are added to the wells. The reactions are terminated and a green color develops in proportion to the amount of inorganic phosphate released by the coupling phosphatase. The absorbance of the color at 620 nm is measured.