Coupling Rates for 20 Minute Kinase Reactions

The following table lists the coupling rates for 20 minute kinase reactions coupled with Recombinant Mouse CD39L2. The coupling rates are for 50 μL reactions carried out at room temperature in a buffer consisting of 25 mM HEPES, 10 mM CaCl₂, 10 mM MgCl₂, 150 mM NaCl, pH 7.0. The coupling rates were calculated using a specific rate constant of 97.78 nmol/min/μg/mM.

NOTE: An * represents the optimal range for coupling rates in order to achieve the maximum signal-to-noise ratio for the phosphatase-coupled kinase reaction.

For more detailed technical information, please also see: Wu, Z.L. (2011) PLoS ONE 6(8):e23172.

Coupling Rates for 20 Minute Kinase Reactions

ATP (μM)	CD39L2 (μg)
ATP (μM)	0.025 μg	0.050 μg	0.075 μg	0.100 μg	0.125 μg	0.150 μg	0.175 μg	0.200 μg
50 μM	0.323	0.515*	0.634	0.712	0.765	0.802	0.830	0.851
100 μM	0.309	0.498*	0.618	0.697	0.752	0.791	0.820	0.842
150 μM	0.299	0.486*	0.606	0.686	0.742	0.783	0.813	0.836
200 μM	0.291	0.475*	0.596*	0.677	0.734	0.776	0.806	0.830
250 μM	0.285	0.467*	0.587*	0.669	0.727	0.769	0.801	0.825
300 μM	0.279	0.460*	0.580*	0.662	0.721	0.764	0.796	0.821
400 μM	0.269	0.447*	0.567*	0.650	0.710	0.754	0.787	0.813
500 μM	0.262	0.436*	0.556*	0.640	0.700	0.745	0.779	0.806
600 μM	0.255	0.427*	0.546*	0.631	0.692	0.738	0.773	0.800
700 μM	0.249	0.419*	0.537*	0.622	0.684	0.731	0.766	0.794
800 μM	0.244	0.411*	0.530*	0.615	0.677	0.724	0.761	0.789
900 μM	0.239	0.405*	0.522*	0.608	0.671	0.718	0.755	0.784
1000 μM	0.234	0.399	0.516*	0.601	0.665	0.713	0.750	0.779
1100 μM	0.230	0.393	0.510*	0.595*	0.659	0.708	0.745	0.775
1200 μM	0.227	0.388	0.504*	0.590*	0.654	0.703	0.741	0.771
1300 μM	0.223	0.383	0.499*	0.584*	0.649	0.698	0.736	0.767
1400 μM	0.220	0.378	0.493*	0.579*	0.644	0.693	0.732	0.763
1500 μM	0.217	0.374	0.489*	0.574*	0.639	0.689	0.728	0.760
1600 μM	0.214	0.370	0.484*	0.570*	0.635	0.685	0.725	0.756
1700 μM	0.212	0.366	0.480*	0.565*	0.631	0.681	0.721	0.753
1800 μM	0.209	0.362	0.476*	0.561*	0.626	0.677	0.717	0.749
1900 μM	0.207	0.358	0.472*	0.557*	0.623	0.674	0.714	0.746
2000 μM	0.204	0.355	0.468*	0.553*	0.619	0.670	0.711	0.743
2100 μM	0.202	0.352	0.464*	0.549*	0.615	0.667	0.707	0.740
2200 μM	0.200	0.349	0.460*	0.546*	0.612	0.663	0.704	0.737
2300 μM	0.198	0.346	0.457*	0.542*	0.608	0.660	0.701	0.735
2400 μM	0.196	0.343	0.454*	0.539*	0.605	0.657	0.698	0.732
2500 μM	0.194	0.340	0.451*	0.536*	0.602	0.654	0.696	0.729
2600 μM	0.193	0.337	0.448*	0.532*	0.599*	0.651	0.693	0.727
2700 μM	0.191	0.335	0.445*	0.529*	0.596*	0.648	0.690	0.724
2800 μM	0.189	0.332	0.442*	0.526*	0.593*	0.645	0.687	0.722
2900 μM	0.188	0.330	0.439*	0.523*	0.590*	0.642	0.685	0.719
3000 μM	0.186	0.327	0.436*	0.521*	0.587*	0.640	0.682	0.717

Coupling Rates for 20 Minute Kinase Reactions

Coupling Rates for 20 Minute Kinase Reactions

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