Antifade Reagents Fluorescent Probes and Dyes

Photobleaching is a fundamental limitation of cell microscopy, reducing the number of photons emitted from a fluorophore molecule, and causing intermittent, fluctuating emission known as blinking. Blinking is caused when a fluorophore randomly enters transient, nonfluorescent states.

Effects of photobleaching can be moderated by reducing the intensity, frequency and duration of the excitation light. However, these steps limit the sensitivity of the experiment. Another approach is to increase ...