Single Cell Multiomics
Single cell analysis technologies are rapidly changing our understanding of biology. Single cell transcriptomics, genomics, and proteomics are becoming common tools for developing high-dimensional data sets that are increasingly used for modeling biology and disease using machine learning and AI. These approaches will continue to become more commonly used, and the demand for scaling these assays will continue to increase.
Single Cell Multiomics with the Pala Single Cell Sorter and Dispenser
Workflow efficiency for plate-based single cell RNA/DNA sequencing & proteomics methods are enhanced through single cell deposition with the Pala. Cells can be selected and sorted with fluorescent markers and viability stains using gentle, high accuracy single cell dispensing to achieve higher yields of single cell measurements with high fidelity.
Pala Benefits Include:
- Accurate single cell dispensing in 96 or 384 well format in as little as 2 min/plate
- Up to 11 fluorescence channels using 2 excitation lasers (405/488 or 488/561)
- Gentle on sensitive cell types (<2 psi / 13.8 kPa sorting pressure)
- 1 uL/cell per well dispensing volume
- Fast start-up and shutdown procedures (<5 min each)
High Single Cell Dispensing Accuracy
Single cell dispensing accuracy (1 cell/well) as determined by plate-based imaging. HEK293, CHO, and A549 were dispensed with Pala or Limiting Dilution pipetting (~60 x 96 well plates). For simplicity, Limiting Dilution data is displayed as an average across all cell lines.
Improved Single Cell Integrity and Read Quality in scRNA-seq
Cells were sorted with FACS or with R&D Systems Dispensing Technology and single cells sequenced. R&D's gentle dispensing technology showed markedly higher numbers of reads and genes detected per cell (post-filtering), higher mapping rates, and lower fractions of mitochondrial reads.
Low Induction of Cellular Stress Expression Markers Compared to FACS
Comparison of stress related gene expression markers from cells sorted with traditional FACS vs. R&Ds gentle dispensing technology. Cells sorted with FACS show altered expression of stress markers like Ubiquitin C (Ubc) likely due to the high sorting pressure.
Related Products
| Product | Catalog Number |
|---|---|
| Pala Cell Sorter and Single Cell Dispenser | NI006/NI007 |
| Single Cell Microfluidic Cartridge Kit | NC003 |
Unlocking Innovation from a Single Cell
In this app note, learn more about the Pala's single cell isolation capabilities along with its high preservation of colony outgrowth rates and stemness marker expression in iPSCs.
Identify more Diverse Cell Types in 10x Genomics Workflows
Uncover how the Pala was used to improve the quality of 10x Genomics single cell RNA sequencing data through gentle single nuclei sorting.
Adding Plate-based Single-cell Sequencing to your Core Lab Capabilities
Discover how Pala can be incorporated into single cell whole genome NGS methods using the QIAseq UPX Single Cell DNA Library Kit for NGS library preparation, enabling ultra‑plexed sequencing.