A33/GPA33 Antibody (23GB5910)

Novus Biologicals | Catalog # NBP3-50438

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB5910
View all A33 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human A33/GPA33 (Uniprot: Q99795)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for A33/GPA33 Antibody (23GB5910)

A33/GPA33 Antibody (23GB5910)

Immunocytochemistry/ Immunofluorescence: A33/GPA33 Antibody (23GB5910)[NBP3-50438] -

Immunocytochemical staining of HeLa cells with A33/GPA33 antibody (1:1,000). Nuclei were stained blue with DAPI; A33/GPA33 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 um.
A33/GPA33 Antibody (23GB5910)

Western Blot: A33/GPA33 Antibody (23GB5910)[NBP3-50438] -

Western blotting analysis using A33/GPA33 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for A33/GPA33 Antibody (23GB5910)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: A33

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Gene Symbol

GPA33

Additional A33 Products

Product Documents for A33/GPA33 Antibody (23GB5910)

Certificate of Analysis

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Product Specific Notices for A33/GPA33 Antibody (23GB5910)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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