Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92626

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Adenine Nucleotide Translocase 1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 40-298 of human Adenine Nucleotide Translocase 1 (NP_001142.2). HASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92626] -

Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92626] - Immunofluorescence analysis of HeLa cells using Adenine Nucleotide Translocase 1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92626] -

Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92626] - Immunofluorescence analysis of PC-12 cells using Adenine Nucleotide Translocase 1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Western Blot: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92626] -

Western Blot: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92626] - Western blot analysis of various lysates, using Adenine Nucleotide Translocase 1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenine Nucleotide Translocase 1

Adenine Nucleotide Translocase 1 (ANT1) catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane. It is is a key component of the mitochondrial permeability transition pore (PT pore) complex and is also one of the most abundant proteins in the mitochondrion making up approximately 10% of all mitochondrial proteins. Originally described as an ADP/ATP exchange factor, ANT1 has also been shown to be required for bax mediated apoptosis. This is effected by binding of bax to residues 105-156 of ANT1, presumably resulting in maintaining the PT pore in an open conformation. Interaction with bax is not required for ANT1 pro-apoptotic activity as overexpression of the ANT1 protein also leads to the induction of apoptosis.

Alternate Names

AAC1, Adenine nucleotide translocator 1, ADP, ADP/ATP translocase 1, ANT 1, ANT1adenine nucleotide translocator 1 (skeletal muscle), ATP carrier protein 1, ATP carrier protein, heart/skeletal muscle, ATP carrier protein, heart/skeletal muscle isoform T1, heart/skeletal muscle ATP/ADP translocator, PEO2, PEO3, solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 4, Solute carrier family 25 member 4, T1ANT

Gene Symbol

SLC25A4

Additional Adenine Nucleotide Translocase 1 Products

Product Documents for Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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