Adenosine Deaminase/ADA Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98771

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Adenosine Deaminase/ADA Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Adenosine Deaminase/ADA.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Adenosine Deaminase/ADA Antibody - Azide and BSA Free

Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98771]

Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98771]

Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Anti-Adenosine Deaminase/ADA rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 40 kDa
Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP2-98771]

Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP2-98771]

Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Adenosine Deaminase/ADA was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-Adenosine Deaminase/ADA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Adenosine Deaminase/ADA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa
Adenosine Deaminase/ADA Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP2-98771] -

Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Immunofluorescence staining of Adenosine Deaminase/ADA in A549 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98771 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for Adenosine Deaminase/ADA Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenosine Deaminase/ADA

Adenosine deaminase is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and from the turnover of nucleic acids in tissues. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Inosine can then be deribosylated (removed from ribose) by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine. Mutations in the gene for adenosine deaminase causing it to not be expressed are one cause of severe combined immunodeficiency (SCID). Mutations causing it to be overexpressed are one cause of hemolytic anemia. There is some evidence that a different allelle (ADA2) may lead to autism. There are 2 isoforms of ADA: ADA1 and ADA2. ADA1 is found in most body cells, particularly lymphocytes and macrophages, where it is present not only in the cytosol but also as the ecto- form on the cell membrane attached to a protein called CD26. ADA2 has only been found in the macrophage where it co-exists with ADA1 where the two isoforms regulate the ratio of adenosine to deoxyadenosine to potentiate the killing of parasites. ADA2 is the predominant form present in human plasma and is increased in many diseases, particularly those associated with the immune system: for example rheumatoid arthritis, psoriasis and sarcoidosis. The plasma AD2 isoform is also increased in most cancers.

Long Name

Adenosine Aminohydrolase

Alternate Names

ADA, ADA1

Gene Symbol

ADA

Additional Adenosine Deaminase/ADA Products

Product Documents for Adenosine Deaminase/ADA Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Adenosine Deaminase/ADA Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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