Albumin Antibody (5B11) - BSA Free

Novus Biologicals | Catalog # NBP2-23495

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B11

Format

BSA Free
View all Albumin Primary Antibodies »

Product Specifications

Immunogen

Native HSA isolated from human plasma adsorbed onto aluminum hydroxide gel

Epitope

Clone 01 differs from 192-02 and Clone 05, but overlap as determined by inhibition ELISA.

Reactivity Notes

Human

Specificity

Clone 5B11 is specific for native and denatured human serum albumin (HSA)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Albumin Antibody (5B11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
Clone 5B11 reacts strongly with native and denatured HSA. A strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiter well, and also in sandwich ELISA in combination with a polyclonal antibody against HSA. In Western blotting after SDS-PAGE, Clone 5B11 reacts with native and denatured HSA in non-reduced form only.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Albumin

Human serum albumin (HSA) consists of a single polypeptide chain of 584 amino acids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa

Alternate Names

ALB

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Albumin Antibody (5B11) - BSA Free

Certificate of Analysis

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Product Specific Notices for Albumin Antibody (5B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Phase Proteins

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