Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

Novus Biologicals | Catalog # NBP3-23187

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M562
View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies »

Product Specifications

Immunogen

Clone M562 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human Aldehyde Dehydrogenase 1-A1/ALDH1A1. [UniProt# P00352]

Specificity

This antibody antibody detects a 55 kDa protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant Aldehyde Dehydrogenase 1-A1/ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562) [NBP3-23187] -

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562) [NBP3-23187] - Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1 (lane 6). The blot was probed with mouse monoclonal Aldehyde Dehydrogenase 1-A1/ALDH1A1 M562 (lanes 1-6) at a dilution of 1:1,000.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562) [NBP3-23187] -

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562) [NBP3-23187] - Immunocytochemical labeling of Aldehyde Dehydrogenase 1-A1/ALDH1A1 in aldehyde fixed and NP-40 permeabilized human A431 cells. The cells were labeled with mouse monoclonal anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 (NBP3-23187). The antibody was detected using goat anti-mouse DyLight (R) 594.

Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Gene Symbol

ALDH1A1

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

Certificate of Analysis

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Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M562)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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