AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

Novus Biologicals | Catalog # NBP3-33579

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2O0P9
View all AMPK alpha 1/2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around T183/T172 of human AMPK alpha 1/2 (NP_006242.5/NP_006243.2).

Sequence:
LRTSC

Modification

p Thr172, p Thr183

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] -

Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] - Western blot analysis of lysates from C2C12 cells, using AMPK alpha 1/2 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated by mHGF(50ng/uL).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] -

Western Blot: AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] - Western blot analysis of lysates from C6 cells, using AMPK alpha 1/2 Rabbit mAb at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9) [NBP3-33579] -

Analysis of lysates from 293T cells using Phospho-AMPK alpha 1-T183+AMPK alpha 2-T172 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. 293T cells were treated with CA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Applications for AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK alpha 1/2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic a subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms (alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations capable of forming complexes. The catalytic a subunit of AMPK is activated allosterically by AMP and by phosphorylation via the AMPK kinase LKB1. Active AMPK downregulates anabolic pathways such as fatty acid, triglyceride, and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation. AMPK's role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase alpha 1/2

Alternate Names

AMPK, AMPKa1, PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Gene Symbol

PRKAA1

Additional AMPK alpha 1/2 Products

Product Documents for AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AMPK alpha 1/2 [p Thr172, p Thr183] Antibody (2O0P9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

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