Recombinant Feline Angiopoietin-1 Protein

Novus Biologicals | Catalog # NBP3-57475

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all Angiopoietin-1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 122 aa of Feline Angiopoietin-1.

Source: Yeast

Amino Acid Sequence: QDDSRYKHFL TQHYDAKPKG RNDRYCESMM ERRGLTTPCK DTNTFIHGNK GSIKAICGNK NGNPYREALR LSKSPFQITT CRHVGGSPRP PCRYRATPGF RHIAVACENG LPVHFDESFF RP (122)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57475
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Recombinant Feline Angiopoietin-1 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Feline Angiopoietin-1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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