Bad [p Ser112] Antibody (SR1363) - BSA Free

Name: Bad [p Ser112] Antibody (SR1363) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-21589
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-21589

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1363 expressed in HEK293

Format

BSA Free

View all Bad Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-Bad [Ser112] (Uniprot #: Q92934)

Modification

p Ser 112

Specificity

Detects endogenous levels of total phospho-Bad [Ser112]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Bad [p Ser112] Antibody (SR1363) - BSA Free (NBP3-21589) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Bad [p Ser112] Antibody (SR1363) - BSA Free

Western Blot: Bad [p Ser 112] Antibody (SR1363) [NBP3-21589] -

Western Blot: Bad [p Ser 112] Antibody (SR1363) [NBP3-21589] - Western blot analysis using NBP3-21589 in (1) HeLa cell lysate; (2) HeLa cell treated with Calcyculin A lysate.

Applications for Bad [p Ser112] Antibody (SR1363) - BSA Free

Application

Recommended Usage

Immunoprecipitation

1:20

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

Additional Bad Products

Product Documents for Bad [p Ser112] Antibody (SR1363) - BSA Free

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Product Specific Notices for Bad [p Ser112] Antibody (SR1363) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.