Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all BLMH/Bleomycin Hydrolase Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse BLMH/Bleomycin Hydrolase (Accession#: NP_848760.1; Asn 2-Glu 455)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for BLMH/Bleomycin Hydrolase Antibody - Azide and BSA Free

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334]

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334]

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334] - Anti-BLMH/Bleomycin Hydrolase rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa
Immunoprecipitation: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334]

Immunoprecipitation: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334]

Immunoprecipitation: BLMH/Bleomycin Hydrolase Antibody [NBP2-98334] - Mouse BLMH/Bleomycin Hydrolase was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 0.5 ul anti-Mouse BLMH/Bleomycin Hydrolase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse BLMH/Bleomycin Hydrolase rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa

Applications for BLMH/Bleomycin Hydrolase Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLMH/Bleomycin Hydrolase

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]

Alternate Names

BMH

Gene Symbol

BLMH

Additional BLMH/Bleomycin Hydrolase Products

Product Documents for BLMH/Bleomycin Hydrolase Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BLMH/Bleomycin Hydrolase Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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