BMP-6 Antibody (74219.11) - Azide and BSA Free

Novus Biologicals | Catalog # NB120-10859

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 74219.11

Format

Azide and BSA Free
View all BMP-6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length protein (Human) expressed in CHO cells.

Localization

Secreted

Specificity

This shows less than 5 % cross-reactivity with recombinant human BMP5 and BMP7 and no cross-reactivity with recombinant human BMP2 and BMP4 under reducing conditions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for BMP-6 Antibody (74219.11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use at a concentration of 0.5 - 1.0 ug/ml. The detecton limit for recombinant human BMP-6 is approximately 6.2 ng/well.Inhib: Use at a concentration of 1 - 3 ug/ml.WB: Use at a concentration of 1 - 20 ug/ml. The detection limit for recombinant human BMP6 is approximately 20 ng/lane under non-reducing conditions.Anti-Human Bone Morphogenetic Protein 6 (BMP6) is measured by its ability to neutralize the bioactivity of BMP6 in osteoblast MC3T3-E1 cells. Recombinant human BMP6 is added to various concentrations of the antibody for 1 hour at 37. This antibody has neutralizing activity at 0.5-2 ug/ml in an assay using ATDC-5 cells, which indicates that it has specificity to an epitope of the target which neutralizes its functional activity.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 5% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMP-6

Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues.

Long Name

Bone Morphogenetic Protein 6

Alternate Names

BMP6, Vgr-1

Entrez Gene IDs

654 (Human)

Gene Symbol

BMP6

UniProt

P22004

Additional BMP-6 Products

Product Documents for BMP-6 Antibody (74219.11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BMP-6 Antibody (74219.11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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