Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-17990

Novus Biologicals
Discontinued Product
NBP3-17990 has been discontinued. View all Carboxypeptidase B1/CPB1 products.

Key Product Details

Sample Type & Volume Required Per Well

Serum ([Sample Volume])

Sensitivity

5.30 pg/mL

Assay Range

6.25-400 pg/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

Recognizes both recombinant and natural Human Ferritin light chain

精度

Intra-Assay Precision (Precision within an assay) %CV 9.7

Inter-Assay Precision (Precision between assays) %CV 10

Recovery for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

Recovery

83 -93%

Scientific Data Images for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

ELISA: Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric) [NBP3-17990]

ELISA: Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric) [NBP3-17990]

ELISA: Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric) [NBP3-17990] - Standard Reference Curve

Kit Contents for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

  • Color Reagent A
  • Color Reagent B
  • Dilution Buffer Concentrate
  • Human Carboxypeptidase B1 / CPB1 Detecion Antibody
  • Human Carboxypeptidase B1 / CPB1 Microplate
  • Human Carboxypeptidase B1 / CPB1 Standard
  • Stop Solution
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carboxypeptidase B1/CPB1

Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates. This reagent is suited to enable an immunological appraoch to the study of protein and peptide structure.

Alternate Names

CPB1, PASP, PCPB

Gene Symbol

CPB1

Additional Carboxypeptidase B1/CPB1 Products

Product Documents for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

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FAQs for Human Carboxypeptidase B1/CPB1 ELISA Kit (Colorimetric)

Showing  1 - 22 FAQs Showing All
  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

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