Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG1 Clone # 746109
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Caspase-8 synthetic peptide
CGIPVETD
Accession # Q14790

Specificity

Detects human cleaved Caspase-8 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Caspase-8 Antibody (746109) [Janelia Fluor® 525]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Caspase-8

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (746109) [Janelia Fluor® 525]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Caspase-8 Antibody (746109) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Caspase-8 Antibody (746109) [Janelia Fluor® 525]

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    • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
      A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail