CDK8 Antibody (6E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001024-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6E5

Format

Azide and BSA Free
View all CDK8 Primary Antibodies »

Product Specifications

Immunogen

CDK8 (NP_001251, 375 a.a. ~ 464 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

CDK8 - cyclin-dependent kinase 8

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDK8 Antibody (6E5) - Azide and BSA Free

Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02] - CDK8 monoclonal antibody (M02), clone 6E5. Analysis of CDK8 expression in NIH/3T3.
Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02] - CDK8 monoclonal antibody (M02), clone 6E5. Analysis of CDK8 expression in PC-12.
Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02] - CDK8 monoclonal antibody (M02), clone 6E5 Analysis of CDK8 expression in Hela S3 NE.
Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02] - CDK8 monoclonal antibody (M02), clone 6E5. Analysis of CDK8 expression in Raw 264.7.
Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02]

Western Blot: CDK8 Antibody (6E5) [H00001024-M02] - CDK8 monoclonal antibody (M02), clone 6E5. Analysis of CDK8 expression in Jurkat.
Sandwich ELISA: CDK8 Antibody (6E5) [H00001024-M02] - Detection limit for recombinant GST tagged CDK8 is approximately 0.03ng/ml as a capture antibody.

Applications for CDK8 Antibody (6E5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDK8

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Long Name

Cyclin-dependent Kinase 8

Alternate Names

Protein kinase K35

Entrez Gene IDs

1024 (Human)

Gene Symbol

CDK8

OMIM

603184 (Human)

UniProt

NP_001251 (Human)

Additional CDK8 Products

Product Documents for CDK8 Antibody (6E5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDK8 Antibody (6E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail