CHD7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55672

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHD7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ALNLSVPRQRRRRRRKIEIEAERAAKRRNLMEMVAQLRESQVVSENGQEKVVDLSKASREATSSTSNFSSLSSKFILPNVSTPVSDAFKTQMEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CHD7 Antibody - BSA Free (NBP2-55672) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CHD7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CHD7 Antibody [NBP2-55672]

Immunocytochemistry/ Immunofluorescence: CHD7 Antibody [NBP2-55672]

Immunocytochemistry/Immunofluorescence: CHD7 Antibody [NBP2-55672] - Staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.
CHD7 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: CHD7 Antibody - BSA Free [NBP2-55672]

Chromatin Immunoprecipitation-exo-Seq: CHD7 Antibody - BSA Free [NBP2-55672]

ChIP-Exo-Seq composite graph for Anti-CHD7 (NBP2-55672) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for CHD7 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD7

Human CHD7 is a chromodomain helicase DNA-binding protein and mutations in the CHD7 gene are a major cause of CHARGE syndrome. CHARGE syndrome is a well characterized multiple-malformation syndrome with distinctive diagnostic critera. Anomalies include ocular coloboma, choanal atresia, cranial nerve defects, distinctive external and inner ear abnormalities, hearing loss, cardiovascular malformations, urogenital anomalies, and growth retardation.

Long Name

Chromodomain Helicase DNA Binding Protein 7

Alternate Names

ATP-dependent helicase CHD7, CHD-7, chromodomain helicase DNA binding protein 7, chromodomain helicase DNA binding protein 7 isoform CRA_e, chromodomain-helicase-DNA-binding protein 7, EC 3.6.1, EC 3.6.4.12, FLJ20357, FLJ20361, IS3, KIAA1416KAL5

Gene Symbol

CHD7

Additional CHD7 Products

Product Documents for CHD7 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CHD7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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