Key Product Details

Species Reactivity

Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 292012
View all RGM-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken RGM-A
Pro150-Asn404 (Asn404Asp), predicted
Accession # Q8JG54

Specificity

Detects chicken RGM‑A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human RGM-A or recombinant mouse RGM-A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Chicken RGM‑A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Chicken Repulsive Guidance Molecule A/RGM-A

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RGM-A

RGM-A is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)- anchored axon guidance proteins. It is a disulfide-linked dimer of the N‑terminal and C-terminal RGD-containing segments. RGM-A binds with high affinity to Neogenin.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Q8JG54

Additional RGM-A Products

Product Documents for Chicken RGM‑A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Chicken RGM‑A Antibody

For research use only

Related Research Areas

BMP Family

Citations for Chicken RGM‑A Antibody

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