Collagen IV alpha5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-41417
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
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Product Specifications
Immunogen
Immunized with recombinant Human Collagen IV alpha5 (Uniprot#: P29400; Gly1461-Thr1685 (Uniprot#: P29400)
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for Collagen IV alpha5 Antibody - BSA Free
Western Blot: Collagen IV alpha5 Antibody [NBP3-41417] -
Western Blot: Collagen IV alpha5 Antibody [NBP3-41417] - Sample: Mouse Cerebrum lysate; Primary Ab: 3ug/ml NBP3-41417; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal AntibodyWestern Blot: Collagen IV alpha5 Antibody [NBP3-41417] -
Western Blot: Collagen IV alpha5 Antibody [NBP3-41417] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate Primary Ab: 0.15ug/ml NBP3-41417; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal AntibodyWestern Blot: Collagen IV alpha5 Antibody [NBP3-41417] -
Western Blot: Collagen IV alpha5 Antibody [NBP3-41417] - Sample: Porcine Cerebrum lysate; Primary Ab: 3ug/ml NBP3-41417; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal AntibodyApplications for Collagen IV alpha5 Antibody - BSA Free
Application
Recommended Usage
Western Blot
0.01-3ug/mL
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.01M PBS, pH7.4, 50% glycerol
Format
BSA Free
Preservative
0.05% Proclin 300
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen IV alpha5
Alternate Names
alpha-5 polypeptide, Alport syndrome, CA54, collagen of basement membrane, alpha-5 chain, collagen, type IV, alpha 5, dA149D17.3, dA24A23.1, MGC167109, MGC42377
Gene Symbol
COL4A5
Additional Collagen IV alpha5 Products
Product Documents for Collagen IV alpha5 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Collagen IV alpha5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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