COMMD5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92325

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human COMMD5 (NP_054785.2). MSAVGAATPYLHHPGDSHSGRVSFLGAQLPPEVAAMARLLGDLDRSTFRKLLKFVVSSLQGEDCREAVQRLGVSANLPEEQLGALLAGMHTLLQQALRLPPTSLKPDTFRDQLQELCIPQDLVGDLASVVFGSQRPLLDSVAQQQGAWLPHVADFRWRVDVAISTSALARSLQPSVLMQLKLSDGSAYRFEVPTAKFQELRYSVALVLKEMADLEKRCERRLQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COMMD5 Antibody - Azide and BSA Free

COMMD5 Antibody - Azide and BSA Free

Western Blot: COMMD5 Antibody - Azide and BSA Free [NBP2-92325] -

Western Blot: COMMD5 Antibody - Azide and BSA Free [NBP2-92325] - Western blot analysis of various lysates, using COMMD5 Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
COMMD5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: COMMD5 Antibody - Azide and BSA Free [NBP2-92325] -

Immunocytochemistry/ Immunofluorescence: COMMD5 Antibody - Azide and BSA Free [NBP2-92325] - Immunofluorescence analysis of C6 cells using COMMD5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for COMMD5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COMMD5

Alternate Names

COMM domain containing 5, COMM domain-containing protein 5, FLJ13008, HCARG, HT002, Hypertension-related calcium-regulated gene protein, MGC72046

Gene Symbol

COMMD5

Additional COMMD5 Products

Product Documents for COMMD5 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for COMMD5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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