CoraFluor™ 1, thiol reactive
Tocris Bioscience | Catalog # 8117
Key Product Details
Description
Product Description
CoraFluor™ 1, thiol reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. This product is designed with maleimide functionality to conjugate to cysteines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 1, thiol reactive is compatible with common acceptor dyes such as FAM, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, amine reactive, CoraFluor™ 2, amine reactive, CoraFluor™ 1, Haloalkane, and CoraFluor™ 2, Haloalkane, also available.
Please refer to the protocol for further information on conjugation.
For more information, please see our CoraFluor™ TR-FRET technology flyer .
CoraFluor™ is a trademark of Bio-Techne Corp.
Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254Optical Data
| λabs | 338 nm |
| Closest Laser line | 337 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | No |
| Correction Factor 280 | 0.157 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications for CoraFluor™ 1, thiol reactive
Molecular Weight
Storage
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
References are publications that support the biological activity of the product.
- Payne A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 2022 PMID: 35649410
- Payne Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 2022 PMID: 35298895
- Payne Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 2021 PMID: 34675420
Product Documents for CoraFluor™ 1, thiol reactive
Product Specific Notices for CoraFluor™ 1, thiol reactive
For research use only
Customer Reviews for CoraFluor™ 1, thiol reactive (1)
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Species: Labeling recombinant proteinAssay Type: In VitroVerified Customer | Posted 04/09/2025Make sure to reduce with TCEP, exchange or quench, bubble everything with inert gas; 2 fold molar excess works well.I used CoraFluor-1 to label recombinant human protein with single cysteine for a TR-FRET assay. Since the amount is small, I went with a 2-fold molar excess of the probe per cysteine. I reduced the protein with fresh TCEP, buffer exchanged, and bubbled everything with nitrogen to keep conditions reducing. Labeling worked great. The probe is super bright—I get ~700x signal over buffer blank at 490/10 nm emission using just 5 nM. Overall, really solid performance and great for TR-FRET, even with low amounts.
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