CPT1A Antibody (8Y1J4)

Novus Biologicals | Catalog # NBP3-33364

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 8Y1J4
View all CPT1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).

Sequence:
WDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CPT1A Antibody (8Y1J4) (NBP3-33364) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CPT1A Antibody (8Y1J4)

CPT1A Antibody (8Y1J4)

Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] -

Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
CPT1A Antibody (8Y1J4)

Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] -

Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from wild type (WT) and CPT1A knockdown (KD) HeLa cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
CPT1A Antibody (8Y1J4)

Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] -

Western Blot: CPT1A Antibody (8Y1J4) [NBP3-33364] - Western blot analysis of lysates from MCF7 cells using [KD Validated] CPT1A Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Applications for CPT1A Antibody (8Y1J4)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CPT1A

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Alternate Names

CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21

Gene Symbol

CPT1A

Additional CPT1A Products

Product Documents for CPT1A Antibody (8Y1J4)

Certificate of Analysis

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Product Specific Notices for CPT1A Antibody (8Y1J4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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