csl/RBPJK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33427

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Mouse (97%), Rat (98%), Rhesus Macaque (99%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CSL Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human csl/RBPJK. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for csl/RBPJK Antibody - BSA Free

csl/RBPJK Antibody

Western Blot: csl/RBPJK Antibody [NBP1-33427] -

Western Blot: csl/RBPJK Antibody [NBP1-33427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with csl/RBPJK antibody [C1C3] (NBP1-33427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,
Immunocytochemistry/ Immunofluorescence: csl/RBPJK Antibody [NBP1-33427]

Immunocytochemistry/ Immunofluorescence: csl/RBPJK Antibody [NBP1-33427]

Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] - Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.
Chromatin Immunoprecipitation (ChIP): csl/RBPJK Antibody [NBP1-33427] - Sheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The chromatin buffer was adjusted to 150 mM NaCl, 20 mM Tris-Cl pH 8, 2 mM EDTA, and 1% Triton X-100. Three antibodies, as well as pure pre-immune rabbit IgG control, were added to each IP reaction, consisting of 7 million cells, and incubated overnight with mixing at 4 C. The antibodies were captured with Magna ChIP Protein A+G beads (Millipore), washed with three wash buffers, and the DNA eluted and purified with the iPure v2 kit (Diagenode). The purified DNA was normalized to the input control with qPCR quantification of the insulin exon 1 gene (INS) and an RBPJ binding site in the promoter of HES1. Image from verified customer review.

Applications for csl/RBPJK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
Chip reactivity per customer review.

Reviewed Applications

Read 1 review rated 3 using NBP1-33427 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CSL

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate. determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence

Long Name

Recombining Binding Protein Supressor of Hairless [CBF1, CSL]

Alternate Names

CBF1, IGKJRB1, KBF2, RBP-JK, RBPJ, RBPSUH

Entrez Gene IDs

3516 (Human)

Gene Symbol

RBPJ

UniProt

Q06330

Additional CSL Products

Product Documents for csl/RBPJK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for csl/RBPJK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Dendritic Cell Development
Notch Pathway

Citations for csl/RBPJK Antibody - BSA Free

Customer Reviews for csl/RBPJK Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used csl/RBPJK Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • csl/RBPJK Antibody
    Name: Christopher Cardinale
    Application: Chromatin Immunoprecipitation
    Sample Tested: sheared cross-linked chromatin
    Species: Human
    Verified Customer | Posted 07/12/2018
    Chromatin immunoprecipitation of RBPJ was performed on sheared, cross-linked Jurkat cell chromatin using 3 different antibodies. The negative control amplicon is insulin exon 1 (INS) and the positive control is the HES1 promoter.
    Sheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The chromatin buffer was adjusted to 150 mM NaCl, 20 mM Tris-Cl pH 8, 2 mM EDTA, and 1% Triton X-100. The three indicated antibodies, as well as pure pre-immune rabbit IgG control, were added to each IP reaction, consisting of 7 million cells, and incubated overnight with mixing at 4 C. The antibodies were captured with Magna ChIP Protein A + G beads (Millipore), washed with three wash buffers, and the DNA eluted and purified with the iPure v2 kit (Diagenode). The purified DNA was normalized to the input control with qPCR quantification of the insulin exon 1 gene (INS) and an RBPJ binding site in the promoter of HES1.
    csl/RBPJK Antibody - BSA Free NBP1-33427

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies