Cultrex Synthetic Hydrogel Kit (RUO), 1 mL
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Cultrex Synthetic Hydrogel Kit (RUO), 1 mL Summary
Cultrex Synthetic Hydrogel is a fully defined, synthetic component derived hydrogel formulation developed to further advance 3D organoid and cell culturing. This product is ideal for end users who have development efficiency, process consistency, and compliance driven end goals.Key Benefits
• Improved Culture Consistency: Controlled formulation reduces variables
• Uncompromised Performance: Comparable performance to animal component derived matrices
• Improved Imaging: Non-Fluorescent hydrogel is optimal for high- and super-resolution microscopy methods
• Efficient Harvesting: Synthetic component derived formulation allows for facile organoid and cell extraction with protease digestion
• User-Friendly Format: Kit format provided with validated protocol to support out-of-the-box user experience
Why Use Cultrex Synthetic Hydrogel?
Cultrex Synthetic Hydrogel is a fully defined, synthetic-component derived hydrogel formulation. While animal-component derived formulations have inherent variability, synthetic component derived formulations enable increased variable control for improved consistency and improved compliance for end users trying to reduce animal component risks. Formed through the crosslining of synthetic polymer chains, the soft structure and porosity of hydrogels is able to mimic living tissue environments.
Cultrex Synthetic Hydrogel provides a proprietary formulation that has a storage modulus observed to support 3D cell and tissue organoid cultures in human intestinal, human liver, and human lung applications requiring an extracellular matrix scaffold.
What Comes in the Cultrex Synthetic Hydrogel Kit?
One kit includes 8 vial components to prepare desired hydrogel volume. One kit prepares up to 1 mL of total hydrogel volume. A digital protocol is provided for optimal out-of-the-box customer experience. See Product Datasheet.
Specifications
Gelling Assay - Cultrex Synthetic Hydrogel gels in less than 30 minutes at 37 °C.
Dome Assay Cultrex Synthetic Hydrogel forms and maintains stable 3-D dome structures on cell culture plates.
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Scientific Data
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Differentiation of intestinal organoids in Cultrex Synthetic Hydrogel. Organoids expanded in Cultrex Synthetic Hydrogel exhibit expected cystic morphology, indicative of healthy baseline development. Upon differentiation, the organoids begin to lose their cystic morphology and start forming more specialized, structured tissues. This demonstrates the ability of Cultrex Synthetic Hydrogel to support both healthy initial organoid growth and subsequent differentiation into mature structures.
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Cultrex Synthetic Hydrogel performance compared to basement membrane extracts. Cultrex UltiMatrix BME (Catalog # BME001-05) domes were plated at 10,000 cell per 10 μL hydrogel, and Cultrex Synthetic Hydrogel (Catalog # CSH-RUO) domes were plated at 15,000 cells. After three days in culture, comparable organoid growth is observed.
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Cultrex Synthetic Hydrogel performance compared to competitor basement membrane extracts. Domes using animal component derived matrix from another supplier and Cultrex Synthetic Hydrogel (Catalog # CSH-RUO) were each plated at 10,000 cell per 10 μL hydrogel. Morphologically similar organoid growth is observed after 4 and 5.5 days, respectively.
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Cultrex Synthetic Hydrogel outperforms alternative synthetic matrices. Successful organoid growth observed in Cultrex Synthetic Hydrogel (Catalog # CSH-RUO-01) while organoids encapsulated in a competitor's defined hydrogel showed failed organoid formation and significant cell death by day 4.
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Defined matrix supports organoid growth. Adult airway organoids were cultured in Cultrex Synthetic Hydrogel (Catalog # CSH-RUO-01) with Recombinant Human FGF-10 Heat Stable Protein (Catalog # BT-FGF10HS). Scale bar indicates 1 mm.
FAQs
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