Forskolin

Tocris Bioscience | Catalog # 1099

Adenylyl cyclase activator
Tocris Bioscience

Key Product Details

Description

Adenylyl cyclase activator
View all Adenylyl Cyclase Activators »

Product Description

Forskolin, or coleonol, is a cell-permeable, potent, reversible and rapid activator of adenylyl cyclase, an enzyme that converts ATP to cAMP and pyrophosphate (EC50 = 0.5 μM). In combination with other compounds, forskolin enables chemical reprogramming of mouse embryonic fibroblasts to pluripotent stem cells, without genetic factors (protocol available). In combination with IBMX (Cat. No. 2845), forskolin induces neural differentiation of mesenchymal stem cells by downregulating neuron restrictive silencer factor. In a rat model of Huntington's disease-like neurodegeneration, forskolin also exhibits neuroprotective effects. Hypotensive and vasodilatory agent.

Water-soluble analog, NKH 477 (Cat. No. 1603), and Forskolin synthesized to Ancillary Material Grade also available.

For more information about how Forskolin may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Reprogramming Mouse Embryonic Fibroblasts (MEFs) into Skeletal Muscle Progenitors, Highly Efficient Generation of Chemically Induced Pluripotent Stem Cells (CiPSCs) from Mouse Embryonic Fibroblasts (MEFs).

Scientific Data Images for Forskolin

Adult Stem Cell-derived Liver Organoids

Application of Forskolin in Culture and Characterization of Adult Stem Cell-derived Liver Organoids.

Adult stem cell-derived liver organoids were cultured using Forskolin, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), N-Acetylcysteine (Catalog # 7874), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3 beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

Product Specifications for Forskolin

Molecular Weight

410.51

Formula

C22H34O7

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-5-(Acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1H-naphtho[2,1-b]pyran-1-one

CAS Number

66575-29-9

PubChem ID

47936

InChI Key

OHCQJHSOBUTRHG-KGGHGJDLSA-N

SMILES

C=C[C@](C3)(C)O[C@]2(C)[C@@H](OC(C)=O)[C@@H](O)[C@@]1([H])C(C)(C)CC[C@H](O)[C@@](C)1[C@](O)2C3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.26 25
Ethanol 4.11 10

Preparing Stock Solutions for Forskolin

The following data is based on the product molecular weight 410.51.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 9.74 mL 48.72 mL 97.44 mL
1.25 mM 1.95 mL 9.74 mL 19.49 mL
2.5 mM 0.97 mL 4.87 mL 9.74 mL
12.5 mM 0.19 mL 0.97 mL 1.95 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 918 publications citing the usage of this product.

Product Documents for Forskolin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Forskolin

For research use only

Customer Reviews for Forskolin (6)

4.7 out of 5
6 Customer Ratings
5 Stars
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4 Stars
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Customer Images

Showing  1 - 5 的 6 reviews Showing All
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  • Works well with organoids
    Name: Gautam Kok
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Liver organoids
    Verified Customer | Posted 11/19/2020
    Culturing liver organoids - used to swell the organoids
    Forskolin 1099
  • Jurkat cells treated with Forskolin
    Name: Tahmineh Safaie
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Jurkat
    Verified Customer | Posted 08/04/2020
    Jurkat cells were treated with different concentration of Forskolin for 15 minutes and the cAMP was measured (the cAMP levels are reversely correlated with Luminescent values.)
    Forskolin 1099
  • Validation
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: Sprague-Dawley rats primary neuronal
    Verified Customer | Posted 04/04/2020
    10 μM for 30min
    Forskolin 1099
  • To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Forskolin)
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: ips
    Verified Customer | Posted 12/04/2019
    To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Parnate, 3 mM Chir99021, 1 mM A83-01, and 10 mM Forskolin)
    Forskolin 1099
  • Forskolin increases intracellular cAMP levels
    Name: Niroj Shrestha
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 07/08/2019
    HEK293 cells transfected with cAMP FRET sensor, Epac-S(H187) were perfused in normal tyrode solution and excited alternately at 430 nm and fluorescence was captured at 470 and 535 nm. Forskolin (10 µM) was added at 1 min to activate adenylyl cyclase and increase intracellular cAMP, which is detected as an increase in 470/535 fluorescence ratio.
    Forskolin 1099
  • a potent adenylyl cyclase
    Name: Anonymous
    Species: Rat
    Verified Customer | Posted 02/21/2018
    Forskolin activates adenylyl cyclase and increases intracellular cAMP. We used at 10 microM in cultured astrocytes to increase intracellular cAMP level.

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