Cyclin E2 (CCNE2) is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (aa 141-231 and aa 241-361). An alternate splice form lacks 45 amino acids within the first cyclin box fold.
Rat Cyclin E2 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-75111
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
18.75 pg/mL
Assay Range
31.25 - 2000 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat CCNE2 in samples. No significant cross-reactivity or interference between Rat CCNE2 and analogues was observed.
精度
Intra-Assay Precision (Precision within an assay) CV% 4.95%
Inter-Assay Precision (Precision between assays) CV% 5.25%
Recovery for Rat Cyclin E2 ELISA Kit (Colorimetric)
Recovery
86-106%
Linearity
Scientific Data Images for Rat Cyclin E2 ELISA Kit (Colorimetric)
ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] -
ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] - Standard Curve ReferenceKit Contents for Rat Cyclin E2 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Cyclin E2
Alternate Names
CCNE2, CYCE2
Gene Symbol
CCNE2
Additional Cyclin E2 Products
Product Documents for Rat Cyclin E2 ELISA Kit (Colorimetric)
Product Specific Notices for Rat Cyclin E2 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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