Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.
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Recombinant Canine Cystatin C Protein
Novus Biologicals | Catalog # NBP3-57602
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Key Product Details
Applications
Western Blot, ELISA, Cell Culture
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Product Specifications
Description
A recombinant protein corresponding to 125 aa of Canine Cystatin C.
Source: Yeast
Amino Acid Sequence: ASPGAGRRGS RPGAVGGAVD ADVGEEGVQQ ALDFAVREYN RASNDAYHSR ALRVLRARKQ VVSGMNYFLE VEIGRTRCTK SQPNLDNCPF HDQPHLMRKT LCSFQIYTVP WLGKTSLVKS SCQDV (125)
Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.
Purity
>98%, by SDS-PAGE
Endotoxin Level
No affinity tags & naturally endotoxin-free
Predicted Molecular Mass
13.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))
ELISA Standard (Matched Pair) (ELISA(Sta))
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation, and Storage
NBP3-57602
| Formulation | Lyophilized in 1-5X PBS containing 10% Trehalose |
| Preservative | 10% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Reconstitute in sterile PBS with at least 0.1% carrier protein |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Calculators
Background: Cystatin C
Alternate Names
ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene Symbol
CST3
Additional Cystatin C Products
Product Documents for Recombinant Canine Cystatin C Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Recombinant Canine Cystatin C Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- A Guide to the Colony Forming Cell Assay: Methods and Tips
- Cellular Response to Hypoxia Protocols
- Cultrex UltiMatrix BME Protocol
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- Culturing Rat Cortical Stem Cells: Expansion using the Neurosphere System
- Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells
- ELISA Sample Preparation & Collection Guide
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- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Protocol for CEPT Cocktail Kit
- Protocol for Cell Counting Kit-8
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- Protocol for Culturing Embryonic Rat Spinal Motor Neurons
- Protocol for Culturing Rat Hippocampal Neurons
- Protocol for Culturing Rat Microglia
- Protocol for MortaLIVE™ Non-Toxic Dye
- Protocol for the Differentiation and Characterization of Human Th1 Cells
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Rat Cortical Stem Cell Culture Expansion Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Pre-B Colony Forming Cell (CFC) Assay Using Methylcellulose-based Media
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Use of Cultrex Basement Membrane Extract to Improve Take and Growth of Xenografts in Mice
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
