Cytokeratin 5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-95276

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Cytokeratin 5 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 5.

Specificity

Human Cytokeratin 5/KRT5

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cytokeratin 5 Antibody - Azide and BSA Free

Western Blot: Cytokeratin 5 Antibody [NBP2-95276]

Western Blot: Cytokeratin 5 Antibody [NBP2-95276]

Western Blot: Cytokeratin 5 Antibody [NBP2-95276] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)
Cytokeratin 5 Antibody Immunoprecipitation: Cytokeratin 5 Antibody [NBP2-95276]

Immunoprecipitation: Cytokeratin 5 Antibody [NBP2-95276]

Cytokeratin 5 was immunoprecipitated from 0.5mg MCF-7 whole cell lysate using NBP2-95276 at a 1:100 dilution and 15 ul of 50% Protein G agarose. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 42 kDa
Cytokeratin 5 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody [NBP2-95276] -

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody [NBP2-95276] - Immunofluorescence staining of Cytokeratin 5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-95276 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for Cytokeratin 5 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 5 Antibody - Azide and BSA Free

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Protocols

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FAQs

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