DAB1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92015

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all DAB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human DAB1 (NP_066566.3). MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAB1 Antibody - Azide and BSA Free

DAB1 Antibody - Azide and BSA Free

Western Blot: DAB1 Antibody - Azide and BSA Free [NBP2-92015] -

Western Blot: DAB1 Antibody - Azide and BSA Free [NBP2-92015] - Western blot analysis of various lysates using DAB1 Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
DAB1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: DAB1 Antibody - Azide and BSA Free [NBP2-92015] -

Immunocytochemistry/ Immunofluorescence: DAB1 Antibody - Azide and BSA Free [NBP2-92015] - Immunofluorescence analysis of SH-SY5Y cells using DAB1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for DAB1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAB1

Alternate Names

disabled (Drosophila) homolog 1, disabled homolog 1, disabled homolog 1 (Drosophila)

Gene Symbol

DAB1

Additional DAB1 Products

Product Documents for DAB1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DAB1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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