DAO Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92892

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all D-Amino Acid Oxidase Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human DAO (NP_001908.3). MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVHSPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILEGKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVNCTGVWAGALQRDPLLQPGRGQIMKVDAPWMKHFILTHDPERGIYNSPYIIPGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERTGFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLSRMPPSHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAO Antibody - Azide and BSA Free

Western Blot: DAO AntibodyAzide and BSA Free [NBP2-92892]

Western Blot: DAO AntibodyAzide and BSA Free [NBP2-92892]

Western Blot: DAO Antibody [NBP2-92892] - Analysis of extracts of Rat kidney, using DAO antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: DAO Antibody - Azide and BSA Free [NBP2-92892]

Immunocytochemistry/ Immunofluorescence: DAO Antibody - Azide and BSA Free [NBP2-92892]

Immunocytochemistry/Immunofluorescence: DAO Antibody [NBP2-92892] - Analysis of L929 cells using DAO at dilution of 1:100. Blue: DAPI for nuclear staining.
DAO Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: DAO Antibody - Azide and BSA Free [NBP2-92892] -

Immunocytochemistry/ Immunofluorescence: DAO Antibody - Azide and BSA Free [NBP2-92892] - Immunofluorescence analysis of rat kidney cells using DAO Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Applications for DAO Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:1000 - 1:5000

Reviewed Applications

Read 1 review rated 4 using NBP2-92892 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: D-Amino Acid Oxidase

The DAO gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adeninedinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactiveon the naturally occ

Long Name

D-Amino Acid Oxidase

Alternate Names

DAAO, DAMOX, DAO, OXDA

Gene Symbol

DAO

Additional D-Amino Acid Oxidase Products

Product Documents for DAO Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DAO Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DAO Antibody - Azide and BSA Free

Customer Reviews for DAO Antibody - Azide and BSA Free (1)

4 out of 5
1 Customer Rating
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  • DAO Antibody
    Name: Süleyman Bozkurt
    Application: Immunocytochemistry
    Sample Tested: hela cell
    Species: Human
    Verified Customer | Posted 12/14/2021
    HeLa FlipIn Cell line, endogenous DAO expression, 1:100 Rabbit, AlexaFluor 488 Green. Red is Tom20 Mouse.
    DAO Antibody - Azide and BSA Free NBP2-92892

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