DAPT

Tocris Bioscience | Catalog # 2634

γ-secretase inhibitor; induces neuronal differentiation; blocks Notch signaling
Tocris Bioscience

Key Product Details

Description

γ-secretase inhibitor; induces neuronal differentiation; blocks Notch signaling
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Product Description

DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical and dopaminergic neurons from hPSCs (see our protocols below). Used in protocols to generate myoblasts from human human iPSCs. DAPT also promotes the formation of cone photoreceptors in retinal organoids.

For more information about how DAPT may be used, see our protocols: Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts, Generating Midbrain Dopaminergic Neurons from hPSCs.

DAPT synthesized to Ancillary Material Grade also available.

Scientific Data Images for DAPT

Using DAPT to differentiate hiPSCs into dopaminergic neurons

Dopaminergic Neurons Derived from hiPSCs using DAPT.

Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT and Purmorphamine (Catalog # 4551, Tocris). Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Product Specifications for DAPT

Molecular Weight

432.46

Formula

C23H26F2N2O4

Storage

Store at +4°C

Purity

≥99% (HPLC)

Chemical Name

(2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester

CAS Number

208255-80-5

PubChem ID

5311272

InChI Key

DWJXYEABWRJFSP-XOBRGWDASA-N

SMILES

CC(C)(C)OC([C@H]([C@]2=CC=CC=C2)NC([C@H](C)NC(CC1=CC(F)=CC(F)=C1)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 43.24 100

Preparing Stock Solutions for DAPT

The following data is based on the product molecular weight 432.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.31 mL 11.56 mL 23.12 mL
5 mM 0.46 mL 2.31 mL 4.62 mL
10 mM 0.23 mL 1.16 mL 2.31 mL
50 mM 0.05 mL 0.23 mL 0.46 mL

Calculators

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Product Documents for DAPT

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DAPT

For research use only

Customer Reviews for DAPT (6)

4.8 out of 5
6 Customer Ratings
5 Stars
83%
4 Stars
17%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 的 6 reviews Showing All
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  • For hPSC culture
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human PSC
    Verified Customer | Posted 06/30/2020
    Make 100µl aliquots and keep them at −20 °C for up to 6 months.
    For hPSC culture, final concentration 10 μM DAPT.
    DAPT 2634
  • works well
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: Hippocampal neuron
    Verified Customer | Posted 03/26/2020
    gamma -secretase (100 nM BMS 299897 and 250 nM DAPT)
    DAPT 2634
  • The hiPSCs were maintained with Essential 8 Flex Medium with DAPT
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: ipsc
    Verified Customer | Posted 02/08/2020
    2.5 μM
    DAPT 2634
  • DAPT succesful for dopaminergic neuron differentiation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 01/23/2020
    Solubilised in DMSO.
    Used successfully for the differentiation of iPSCs into dopaminergic neurons.
    DAPT 2634
  • used in human cell line cultures
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 08/20/2019
    In cell line culture of human cells
  • Works great.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Induced pluripotent stem cells
    Verified Customer | Posted 01/04/2018
    I used this product for differentiation of iPSCs into dopaminergic neurons according to the protocol published in Kriks et al, 2011. I used the concentration 10uM and it works really well.

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Showing  1 - 5 的 6 reviews Showing All