DL-AP5

Tocris Bioscience | Catalog # 0105

Potent and selective NMDA antagonist
Tocris Bioscience

Key Product Details

Description

Potent and selective NMDA antagonist

Alternative Names

DL-APV
View all NMDA Receptor Antagonists »

Product Description

DL-AP5 is a racemic mixture of the D- and L-isomers of AP5, a selective NMDA receptor antagonist that competes with glutamate binding and is commonly used to inhibit NMDA-dependent synaptic plasticity. D-AP5 (Cat. No. 0106) is the more active isomer and displays approximately 52-fold higher potency than the L-isomer, L-AP5 (Cat. No. 0107). In vitro D-AP5 reduces NMDA-induced depolarization of cortical neurons, with no effect on the response to L-Quisqualic acid (Cat. No. 0188) or Kainic acid (Cat. No. 0222). Following spinal injection of D-AP5, NMDA-response is rapidly reduced, with no effect seen on spontaneously active neurons.

D-isomer, L-isomer and sodium salt also available.

Product Specifications for DL-AP5

Molecular Weight

197.13

Formula

C5H12NO5P

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

DL-2-Amino-5-phosphonopentanoic acid

CAS Number

76326-31-3

PubChem ID

1216

InChI Key

VOROEQBFPPIACJ-UHFFFAOYSA-N

SMILES

NC(C(O)=O)CCCP(O)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 1.97 10
1eq. NaOH 19.71 100

Preparing Stock Solutions for DL-AP5

The following data is based on the product molecular weight 197.13.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.07 mL 25.36 mL 50.73 mL
5 mM 1.01 mL 5.07 mL 10.15 mL
10 mM 0.51 mL 2.54 mL 5.07 mL
50 mM 0.10 mL 0.51 mL 1.01 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 344 publications citing the usage of this product.

Product Documents for DL-AP5

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DL-AP5

For research use only

Customer Reviews for DL-AP5 (4)

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Showing  1 - 4 的 4 reviews Showing All
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  • Efficient DL-AP5
    Name: Raj Kumar
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Retina
    Verified Customer | Posted 07/25/2023
    Tested the effect of DL-AP5 on treatment-induced expression of the tested protein in retinal microvessels.
    DL-AP5 0105
  • AP5 action at NMDARs
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Cell Line/Tissue: Hypothalamus
    Verified Customer | Posted 06/21/2023
    Needs stirring to be diluted.
    AP5 was in use as a control pharmacological intervention to prove NMDAR-related nature of the registered whole-cell electrophysiological response. 20 microM/l AP5 supressed the response immediately. Red trace: whole-cell response without AP5; black trace: after AP5 application.
    DL-AP5 0105
  • NMDA antagonist
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 10/02/2018
    Blocks NMDA currents as expected
  • blocks within 5 minutes
    Name: Erik Dustrude
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: amygdala neurons
    Verified Customer | Posted 01/04/2018
    5 minutes perfusion at ~1.5ml/min was sufficient for the full effect.
    50uM used to antagonize NMDA receptors to isolate AMPA current.
    DL-AP5 0105

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