Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DPF2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human DPF2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DPF2 Antibody - BSA Free

Western Blot: DPF2 Antibody [NBP2-97764]

Western Blot: DPF2 Antibody [NBP2-97764]

Western Blot: DPF2 Antibody [NBP2-97764] - Anti-DPF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC-12 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa
Immunoprecipitation: DPF2 Antibody [NBP2-97764]

Immunoprecipitation: DPF2 Antibody [NBP2-97764]

Immunoprecipitation: DPF2 Antibody [NBP2-97764] - DPF2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-DPF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DPF2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa
DPF2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: DPF2 Antibody [NBP2-97764] -

Immunocytochemistry/Immunofluorescence: DPF2 Antibody [NBP2-97764] - Immunofluorescence staining of DPF2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97764 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.

Applications for DPF2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPF2

DPF2 is encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.

Alternate Names

Apoptosis response zinc finger protein, D4, zinc and double PHD fingers family 2BAF45D, MGC10180, Protein requiem, REQBRG1-associated factor 45D, requiem, apoptosis response zinc finger, ubi-d4, UBID4requiem, apoptosis response zinc finger gene, zinc finger protein ubi-d4

Gene Symbol

DPF2

Additional DPF2 Products

Product Documents for DPF2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DPF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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